Three-dimensional Fib-Sem reconstruction of microtubule-organelle interaction in whole primary mouse beta cells

The publication from 2021 is available here.

Data

The binned data and segmentation of the cells can be downloaded from here.

Videos

Videos can be viewed and downloaded from here.

Organelle-specific segmentation, spatial analysis, and visualization of volume electron microscopy datasets

The first preprint version of the protocol describing all steps involved in the scientific publication linked above is available here.

CellSketch

Most up to date documentation on CellSketch, the part of the protocol dealing with spatial analysis and visualization, is available here.